Journal: Antibiotics

Article Title: Antarctic Marine Bacteria as a Source of Anti-Biofilm Molecules to Combat ESKAPE Pathogens

doi: 10.3390/antibiotics12101556

Figure Lengend Snippet: Antimicrobial susceptibility of clinical and reference strains of S. aureus .

Article Snippet: S. aureus, ATCC 6538P and ATCC 29213 reference strains (originally isolated from a wound), which are commonly used for susceptibility disc testing, quality control, and drug discovery research.

Techniques:

Journal: Antibiotics

Article Title: Antarctic Marine Bacteria as a Source of Anti-Biofilm Molecules to Combat ESKAPE Pathogens

doi: 10.3390/antibiotics12101556

Figure Lengend Snippet: Effect of SNs from Antarctic bacteria against biofilm formation of different clinical strains and ATCC 6538P and ATCC 29213 reference strains of S. aureus . Panel ( A ): Effect of SNs on biofilm formation. SNs were added to the culture medium at time zero (0 h, pre-adhesion period) and the biofilm was analyzed after overnight incubation. In the ordinate axis, the percentage of bacterial biofilm production is reported. Panel ( B ): Effect of SNs on the mature biofilm. SNs were added to the culture medium after 24 h of biofilm growth (24 h of bacterial culture) and the biofilm was analyzed after overnight incubation. In the ordinate axis, the percentage of residual biofilm is reported. Data are expressed as the percentage of residual biofilm after 24 h of treatment with SNs compared with the control sample. Each data point is composed of three independent experiments, each performed with at least three replicates. Error bars indicate the standard deviations of all the measurements. Statistical difference was determined by Student’s t -test: * p < 0.05; ** p < 0.01, *** p < 0.001 compared with the control.

Article Snippet: S. aureus, ATCC 6538P and ATCC 29213 reference strains (originally isolated from a wound), which are commonly used for susceptibility disc testing, quality control, and drug discovery research.

Techniques: Bacteria, Incubation

Journal: International Wound Journal

Article Title: Selective detection of urease‐producing bacteria on the genital skin surface in patients with incontinence‐associated dermatitis

doi: 10.1111/iwj.14209

Figure Lengend Snippet: An original urea agar medium was evaluated in vitro. A, The absorbance at 620 nm of the urea agar medium was changed in addition to the urease solution in a concentration‐dependent manner. B, An example of photographs of the urea medium agar in addition to the urease solution in each concentration. C, Example of a photograph of the urea medium after the bacterial suspension was added and incubated aerobically at 37°C. Staphylococcus aureus and Proteus mirabilis and Escherichia coli were used as positive and negative controls, respectively. The pH on each agar plate was measured to confirm that urease‐producing bacteria increase the pH on agar surfaces in the presence of urea.

Article Snippet: For the standard strains, Staphylococcus aureus (ATCC 6538P) and Proteus mirabilis (ATCC 12453) and Escherichia coli (ATCC 25922) were used as positive and negative controls, respectively., , After being picked out from frozen stock at −80°C, all strains were cultured aerobically at 37°C in trypticase soy agar with 5% sheep blood (Becton Dickinson, USA).

Techniques: In Vitro, Concentration Assay, Suspension, Incubation, Bacteria